Pyruvate dehydrogenase (PDH) WB Antibody Cocktail ab110416 from Abcam

Product Specs
ItemPyruvate dehydrogenase (PDH) WB Antibody Cocktail
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab110416
Quantity150 UG, 300 UG
Conjugate/TagUnconjugated
ApplicationsCellular Assay
ReactivityCow, Human
TypeQuantitative
TargetMitoProfile® Pyruvate dehydrogenase (PDH)

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) MCU gain- and loss-of-function models define the duality of mitochondrial calcium uptake in heart failurebioRxivApril 22, 2023Joanne F. Garbincius, Timothy S. Luongo, Jonathan P. Lambert, Adam S. Mangold, Emma K. Murray, Alycia N. Hildebrand, Pooja Jadiya, John W. Elrodagainst cyclophilin D (Abcam #ab110324) 1:1,000; and mouse monoclonal against total PDH subunits (Abcam #ab110416) 1:500. Membranes were washed 3 times in TBS-T and incubated for 1.5 hours at room temperature in secondary

(2) Identification of Novel Mitochondrial Pyruvate Carrier Inhibitors by Homology Modeling and Pharmacophore-Based Virtual ScreeningBiomedicinesFebruary 2, 2022Lamees Hegazy, Lauren E. Gill, Kelly D. Pyles, Christopher Kaiho, Sophia Kchouk, Brian N. Finck, Kyle S. McCommis, Bahaa Elgendyanti-phosphorylated PDH E1α S232, S293, and S300 (MilliporeSigma AP1062, AP1063, AP1064), anti-PDH Cocktail (Abcam ab110416), and anti-αTubulin (Sigma T5168); secondary antibodies used were anti-rabbit or anti-mouse IRDYE donkey

(3) Non-Canonical Role of PDK1 as a Negative Regulator of Apoptosis through Macromolecular Complexes Assembly at the ER-Mitochondria Interface in Oncogene-Driven NSCLCCancersAugust 17, 2021De Rosa V, Iommelli F, Terlizzi C, Leggiero E, Camerlingo R, Altobelli GG, Fonti R, Pastore L, Del Vecchio S.(T9026, Sigma-Aldrich), actin (A4700, Sigma-Aldrich), cytochrome C (556433, BD Biosciences) and PDH-E1α (ab110416, Abcam, Cambridge, UK). A cocktail of 5 mAbs against OXPHOS (ab110411, Abcam) recognized the following

(4) Myocardial Lipin 1 knockout in mice approximates cardiac effects of human LPIN1 mutationsJCI InsightMay 10, 2021Kari T. Chambers et al.Santa Cruz Biotechnology Inc.); OXPHOS (MS604-300, Abcam); PDH pSer232 (AP1063, MilliporeSigma); PDH (ab110416, Abcam); cytochrome C (ab110325, Abcam); phospho-PKA substrate (RRXS/T; 9624, Cell Signaling Technology),

(5) Pleiotropic effects of mdivi-1 in altering mitochondrial dynamics, respiration, and autophagy in cardiomyocytesRedox biologySeptember 1, 2020Aishwarya R, Alam S, Abdullah CS, Morshed M, Nitu SS, Panchatcharam M, Miriyala S, Kevil CG, Bhuiyan MS.2775, Cell Signalling), p62 (1:500, GP62-C, Progen), OXPHOS (1:1000, ab110413, Abcam), PDH (1:1000, ab110416, Abcam), OMA1 (1:200, sc-515788, Santacruz), YME1L1 (1:1000, 11510-1-AP, Protein Tech), Atg7 (1:500,

(6) Disturbed Fatty Acid Oxidation, Endoplasmic Reticulum Stress, and Apoptosis in Left Ventricle of Patients With Type 2 DiabetesDiabetesOctober 1, 2019Ljubkovic M, Gressette M, Bulat C, Cavar M, Bakovic D, Fabijanic D, Grkovic I, Lemaire C, Marinovic J.(very-long-chain acyl-CoA dehydrogenase) (sc-376239; Santa Cruz Biotechnology), MitoProﬁle PDH WB Antibody Cocktail (ab110416; Abcam), total OXPHOS human antibody cocktail (against representative subunits of electron transfer

(7) Doxorubicin-induced cardiomyopathy associated with inhibition of autophagic degradation process and defects in mitochondrial respirationScientific reportsFebruary 14, 2019Abdullah CS, Alam S, Aishwarya R, Miriyala S, Bhuiyan MAN, Panchatcharam M, Pattillo CB, Orr AW, Sadoshima J, Hill JA, et al.Anti-β-Actin(1:1000, sc-47778, Santa Cruz Biotechnology), Anti-OXPHOS (1:1000, ab110413, Abcam), Anti-PDH (1:1000, ab110416, Abcam), Anti-LC3B (1:1000, 2775, Cell Signaling Technology), Anti-LAMP2 (1:1000, PA1–655, Invitrogen)

(8) The novel function of tumor protein D54 in regulating pyruvate dehydrogenase and metformin cytotoxicity in breast cancerCancer & metabolismJanuary 1, 2019Zhuang Y, Ly RC, Frazier CV, Yu J, Qin S, Fan XY, Goetz MP, Boughey JC, Weinshilboum R, Wang L.were as follows: TPD54 (Abcam, AB194938), Pyruvate dehydrogenase (PDH) WB antibody cocktail (Abcam, ab110416), NDUFB8 antibody [20E9DH10C12] (Abcam, AB110242), PDH E1α (Abcam, ab110334), PDH E1α (Proteintech,

(9) Cardiac Dysfunction in the Sigma 1 Receptor Knockout Mouse Associated With Impaired Mitochondrial Dynamics and BioenergeticsJournal of the American Heart AssociationOctober 16, 2018Abdullah CS, Alam S, Aishwarya R, Miriyala S, Panchatcharam M, Bhuiyan MAN, Peretik JM, Orr AW, James J, Osinska H, et al.Santa Cruz Biotechnology, Santa Cruz, CA), anti‐OXPHOS (1:1000, ab110413, Abcam, Cambridge, UK), anti‐PDH (1:1000, ab110416, Abcam), anti‐COXIV (1:1000, 4844, Cell Signaling Technology), anti‐Periostin (1:500, NBP1‐300042, Novus

(10) 1α,25-dihydroxyvitamin D3 mitigates cancer cell mediated mitochondrial dysfunction in human skeletal muscle cellsBiochemical and Biophysical Research CommunicationsFebruary 5, 2018Zachary C. Ryan, Theodore A. Craig, Xuewei Wang, Philippe Delmotte, Jeffrey L. Salisbury, Ian R. Lanza, Gary C. Sieck, Rajiv Kumaragainst the following mitochondrial proteins or complexes: VDAC1/Porin (ab15895), PDH antibody cocktail (ab110416), PDH E1-α-subunit, phospho-293 (ab177461), PDP2 (ab133982), PDK4 (ab71240), total OXPHOS (ab110411),

(11) Olanzapine and aripiprazole differentially affect glucose uptake and energy metabolism in human mononuclear blood cellsJournal of Psychiatric ResearchDecember 12, 2016Britta Stapel, Alexandra Kotsiari, Michaela Scherr, Denise Hilfiker-Kleiner, Stefan Bleich, Helge Frieling, Kai G. Kahl

(12) Differential inhibition of PDKs by phenylbutyrate and enhancement of pyruvate dehydrogenase complex activity by combination with dichloroacetateJournal of Inherited Metabolic DiseaseSeptember 1, 2015Rosa Ferriero, Clara Iannuzzi, Giuseppe Manco, Nicola Brunetti-Pierriwere performed with PhosphoDetect anti-PDH-E1α (p-Ser264-α; Calbiochem AP1062), anti-PDH-E1α (Abcam ab110416), and HRP-conjugated secondary antibodies (GE Healthcare) diluted in 5 % milk in tris-buffered saline

(13) Alpha-ketoglutarate dehydrogenase complex-dependent succinylation of proteins in neurons and neuronal cell linesJournal of NeurochemistryJuly 1, 2015Gary E. Gibson, Hui Xu, Huan-Lian Chen, Wei Chen, Travis T. Denton, Sheng ZhangLife Technologies, Grand Island NY); pyruvate dehydrogenase (PDH) antibody cocktail (1:1000 ABCAM Cat # AB110416) which includes anti E2 (69kD), E3bp (54KD), E1a (43.3kD), E1B (39.4 KD) and E1a; fumarate hydratase

(14) Abnormalities in the Tricarboxylic Acid Cycle in Huntington Disease and in a Huntington Disease Mouse ModelJournal of Neuropathology & Experimental NeurologyJune 1, 2015Nima N. Naseri, Hui Xu, Joseph Bonica, Jean Paul G. Vonsattel, Etty P. Cortes, Larry C. Park, Jamshid Arjomand, Gary E. Gibsonpyruvate dehydrogenase WB Antibody Cocktail (PDHC), anti-pyruvate dehydrogenase (PDH) cocktail [Abcam (ab110416) for the following proteins: 69 kDa E2, 54 kDa E3, 43.3 kDa E1α, 39.4 kDaE1β], anti-aconitase 2 (ab71440),

(15) Phenylbutyrate increases pyruvate dehydrogenase complex activity in cells harboring a variety of defectsAnnals of Clinical and Translational NeurologyJuly 1, 2014Rosa Ferriero et al.anti-PDH-E3 (EPR6635). The anti-PDH-E1α, anti-PDH-E1β, anti-PDH-E2, anti-E3BP mixed in a cocktail (#ab110416), and the anti-PDH-E3 (#ab133551) were purchased from Abcam (Cambridge, UK). The antibodies phosphodetect

(16) Phenylbutyrate Therapy for Pyruvate Dehydrogenase Complex Deficiency and Lactic AcidosisScience Translational MedicineMarch 6, 2013Rosa Ferriero, Giuseppe Manco, Eleonora Lamantea, Edoardo Nusco, Mariella I. Ferrante, Paolo Sordino, Peter W. Stacpoole, Brendan Lee, Massimo Zeviani, Nicola Brunetti-PierriAP1064), anti-cytochrome c oxidase IV (COXIV; Cell Signaling Technology Inc.), anti-PDH-E1α (Abcam ab110416) and HRP-conjugated secondary antibodies (GE Healthcare) diluted in 5% milk in TBST and in 1% BSA in

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Pyruvate dehydrogenase (PDH) WB Antibody Cocktail from Abcam

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Pyruvate dehydrogenase (PDH) WB Antibody Cocktail from Abcam

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